052558
gp130 (Glycoprotein 130, CD130, CDw130, IL-6 Receptor Subunit beta, Oncostatin-M Receptor Subunit alpha, Membrane Glycoprotein 130) (Biotin)
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£960.00
- SKU:
- 052558
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Gln23-Glu617 of mouse gp130 expressed in NS0 cells.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Weitere Details:
- Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R. Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities. Applications: Suitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: ELISA (Detection): 0.1-0.4ug/.ml Flow Cytometry: 2.5ug/ml labels 10e6 cells in M1 mouse myeloid leukemia cell line. Western Blot: 0.1ug/ml with recombinant mouse gp130 Fc Chimera. Optimal dilutions to be determined by the researcher. Matched Pair: Capture: C2464-05B Detection: 052558 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice