CD45 (FITC)

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£965.00

SKU:: 052493
Anwendung:: Flow Cytometry
Reinheit:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Glycoproteins isolated from the T cell line, RL-5.
Klon:: L12/201
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Weitere Details:: Immunoprecipitation was only obtained after cross linking antibody to the labelled cell surface. This gave a band of ~200kD. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Neat 1:10; 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: P3.X63.Ag8-U1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.