052475-PE

Siglec 1 (Sialic Acid Binding Ig-like Lectin 1, Sialoadhesin, CD169) (PE)

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£722.00

SKU:: 052475-PE
Anwendung:: Flow Cytometry
Reinheit:: Purified by Protein G affinity chromotography from hybridoma culture supernatant
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Thr20-Leu1639 (predicted) of mouse Siglec-1, expressed in NS0 cells
Klon:: 25E1
Format:: Supplied as a liquid in saline, ≤0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Weitere Details:: Siglec-1, also known as sialoadhesin or CD169, is a 175‑185kD type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells using RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight Optimal dilutions to be determined by the researcher. Excitation: 488nm Emission: 565-605nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Versandbedingungen:: Blue Ice