Hyperoxidized Peroxiredoxin-3

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£856.00

SKU:: 052416
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Hyperoxidized human Prx3 peptide sequence
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Peroxiredoxin-3 (Prx3) is a typical 2-Cys-containing Prx that is involved in the maintenance of cellular thiol redox homeostasis.1 It exists as a homodimer when oxidized and is composed of an N-terminal mitochondrial targeting sequence and a catalytic domain that contains peroxidatic and resolving cysteines (Cp and Cr, respectively).1,2 Reduced Prx3 exists as a monomer and hyperoxidized Prx3 is capable of forming dodecamers.2 Prx3 is ubiquitously expressed and localizes to the mitochondrial matrix.1,3 During the catalytic cycle, hydrogen peroxide oxidizes Cp to form a sulfenic acid intermediate (C-SOH), which reacts with the Cr of the opposite subunit to form a disulfide bond that is subsequently restored to a reduced state by thioredoxin 2 (Trx2).1,4 In the presence of high concentrations of hydrogen peroxide, Cp is oxidized to sulfinic acid (C-SO2H), which can be reduced by sulfiredoxin (Srx), or the irreversible sulfonic acid (C-SO3H) species.1 Knockout of the gene encoding thioredoxin reductase 2 (TrxR2) increases the levels of SO3-Prx3 in isolated mouse embryonic endothelial progenitor cells.4 Hyperoxidized Prx3 (SO2/3-Prx3) is also increased during ferroptosis induced by erastin or RSL3 in vitro.5 Liver levels of SO2/3-Prx3 are increased in mouse models of alcoholic fatty liver disease (AFLD) and non-alcoholic fatty liver disease (NAFLD). Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:200 Western Blot: 1:100-1:200 Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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