052386
C9 (Complement Component 9) (Biotin)
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£544.00
- SKU:
- 052386
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa136-512 of mouse C9 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol. Labeled with Biotin
- Weitere Details:
- Activation of the complement system results in formation of the membrane attack complex (MAC) on the membranes of target cells. The complex is assembled by sequential addition of 1 molecule each of C5b, C6, C7, and C8 and 6 to 16 molecules of the ninth component, C9. DiScipio et al. (1984) screened a human liver cDNA library by the colony-hybridization technique using 2 radiolabelled oligonucleotide probes that correspond to known regions of the C9 aa sequence. The cDNA coding for C9 was sequenced and the protein sequence--537aa in a single polypeptide chain-was derived. The N-terminal half of C9 is predominantly hydrophilic and the C-terminal half is more hydrophobic. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.01-2ug/ml Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice