052086

VEGFR2 (Conserved Site), phosphorylated (Tyr801) (KDR, flk-1, Vascular Endothelial Growth Factor Receptor 2)

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£576.00

SKU:: 052086
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide corresponding to amino acids surrounding Tyr-801 in human VEGFR-2 (coupled to carrier protein).
Format:: Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
Weitere Details:: Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis. Applications: Suitable for use in Western Blot and Immunocytochemistry. Also works for immunofluorescent staining of VEGFR-2 in HUVEC cells. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 detects a 180kD band corresponding to VEGFR-2 in western blots of human endothelial cells treated with pervanadate, and shows strong reactivity toward recombinant human VEGFR-2. This reactivity is removed by akaline phosphatase treatment. In addition, the antibody detects the conserved site in VEGFR-1 (Tyr-794) Immunocytochemistry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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