Protein Gene Product 9.5 (PGP9.5, Ubiquitin CT Hydrolase L1, UCH, UCHL1, Ubiquitin thiolesterase, PARK5)

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£780.00

SKU:: 052082
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated linear peptide corresponding to 18aa from the N-terminal region of human Protein Gene Product 9.5 (UCH-L1).
Format:: Supplied as a liquid in 0.1M Tris-Glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide.
Weitere Details:: Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UniProt: P09936; also known as EC: 3.4.19.12, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1) is encoded by the UCHL1 gene (Gene ID: 7345) in human. UCH-L1 is a member of the peptidase C12 family that can exist as a monomer or homodimer. It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors and is detected in neuronal cell bodies and processes throughout the neocortex. It displays a weak expression in ovary. About 30% of total UCH-L1 is reported to be associated with membranes in brain. Its levels are shown to be down regulated in brains from Parkinson disease and Alzheimer disease patients. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin and is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimeric form may have ATP-independent ubiquitin ligase activity. Mutations in UCHL1 gene are known to cause Parkinson disease 5, a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Some mutations in this gene are also linked to spastic paraplegia 79, where patients display a form of spastic paraplegia, which is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Applications: Suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:20,000 detected Protein Gene Product 9.5 in human and mouse brain tissue lysates. Immunohistochemistry (Paraffin): 1:1000 detected Protein Gene Product 9.5 in human pancreas and human cerebellum tissue sections with heat-induced epitope retrieval Immunofluorescence: 1:1000 detected Protein Gene Product 9.5 in human cerebellum tissue sections. Immunocytochemistry: 1:500 detected Protein Gene Product 9.5 in DU145 and E18 cortical cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice