048730
HIV, gp41 (Glycoprotein 41, Envelope Protein gp41, HIV, HIV Transmembrane Protein gp41, HTLV-III gp41)
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£544.00
- SKU:
- 048730
- Reinheit:
- Purified by Protein A affinity chromatography from animal component-free supernatant. ≥95% (SDS-PAGE)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Human
- Immunogen:
- Lectin-purified gp140 (from ADA.C1 strain).
- Klon:
- A2 (recomb)
- Format:
- Supplied as a liquid in PBS. No preservative added.
- Weitere Details:
- HIV-1 Env consists of two subunits: the surface-exposed glycoprotein gp120, which contains the receptor (CD4) and coreceptor (CCR5 or CXCR4) binding sites, and the transmembrane subunit gp41, which is critical for virus-cell membrane fusion. Sequential binding of HIV-1 to its receptor and coreceptor are the first steps critical for viral entry, whereby CD4 binding triggers a series of conformational changes in Env involving both gp120 and gp41. In gp120, CD4 binding induces rearrangement of the variable loops V1/V2 and formation of the bridging sheet, which allows for repositioning of the V3 loop to facilitate coreceptor binding. In gp41, CD4 binding triggers exposure of the buried N-terminal HR1 domain and its formation into a trimeric coiled-coil structure. Upon receptor and coreceptor binding, the fusion peptide in gp41 is inserted into the cell membrane and the HR1 and HR2 regions rearrange to form the six-helix bundle, which is critical for creation of the fusion pore to enable membrane fusion and efficient viral entry. The antibody clone A2 was found to specifically target the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation. This is a recombinant antibody developed by antibody phage display technology using a human naive antibody gene library. Produced without the use of animals. Purified from HEK 293 cell culture supernatant. Applications: Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice