048679
CXCR5 (C-X-C Chemokine Receptor 5) (APC)
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£787.00
- SKU:
- 048679
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Met1-Pro57 from mouse CXCR5 expressed in E. coli.
- Klon:
- 11D21
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Weitere Details:
- CXCR5 is a 55-60kD member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7Ralpha+ precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57aa N-terminal extracellular region plus a 47aa C-terminal cytoplasmic domain. Over aa1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells. Detects CXCR5 in mouse splenocytes and T follicular helper cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice