048665
MAP2D
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£607.00
- SKU:
- 048665
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Full length recombinant protein corresponding to human MAP2D, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, 5mM sodium azide., 50% glycerol
- Weitere Details:
- Microtubules are 25nm diameter protein rods found in most eukaryotic cells and are associated with a family of proteins called microtubule associated proteins (MAPs). MAPs play a crucial role in the regulation of microtubule dynamics and interactions in vivo. MAP2 was originally named as one of the higher molecular weight MAPs with an SDS-PAGE molecular weight of about 280kDa (1-3). There is a single mammalian MAP2 gene which may generates two high molecular weight proteins of ~280kD on SDS-PAGE named MAP2A and MAP2B and multiple lower molecular weight forms usually named MAP2C and MAP2D which run on SDS-PAGE gels at 60-70kD. The lower molecular weight forms are found in neurons early in development, but as the animal matures they are replaced by the higher molecular weight forms (1,2). MAP2 isoforms are expressed only in neurons in perikarya and dendrites, so MAP2 antibodies are useful for identifying neurons in cell culture and sectioned material (4-8). MAP2C and D contain an “intrinsically unstructured region”, one of the prototypes for this widespread type of protein sequence (9). Since MAP2C and D are expressed earlier in development than MAP2A and B this antibody can be used for monitoring early neuronal development, though it is also useful as a general marker for neurons and dendrites in mature tissues. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilution: Western Blot: 1:10,000-1:20,000 Immunohistochemistry (formalin fixed and paraffin embedded): 1:4000 Immunofluorescence (IC) 1:2000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice