Growth Hormone-Releasing Hormone (GHRH) (Not for Export EU)

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£448.00

SKU:: 047589
Reinheit:: Serum
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Sheep
Immunogen:: Synthetic rat GHRH (1-29)NH2
Format:: Supplied as a lyophilized powder. Reconsitute with 50ul sterile ddH2O.
Weitere Details:: GHRH belongs to the glucagon family produced in the hypothalamus. It is essential for normal linear growth and has important metabolic effects throughout life. GHRH stimulates GH production and release by binding to the GHRH Receptor (GHRHR) on cells in the anterior pituitary. GHRH is released from neurosecretory nerve terminals of arcuate neurons, and is carried by the hypothalamo-hypophyseal portal system to the anterior pituitary gland, where it stimulates growth hormone(GH) secretion by stimulating the growth hormone-releasing hormone receptor. Applications: Suitable for use in ELISA, Immunohistochemistry and RIA. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:15,000 RIA: 1:785,000, this antiserum binds 35% of [125I]-rGRF and can detect 7pg/assay tube. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.