FLAG-Tag (DDDDK Epitope Tag, DYKDDDDK Epitope Tag)

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£581.00

SKU:: 046285
Anwendung:: WB
Reinheit:: Purified by affinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: DYKDDDDK-tagged mouse Langerin
Klon:: L5
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Weitere Details:: The DYKDDDDK tag, commonly referred to as FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins. Applications: Suitable for use in Western Blot. Though not tested, Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation and Immunohistochemistry have been reported in literature. Other applications not tested. Recommended Dilutions: Western Blot: 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.