046193
Complement Component 3/C3a (desArg)
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£672.00
- SKU:
- 046193
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human C3a
- Klon:
- K13/16
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Weitere Details:
- C3, complement component 3, is a 195kD glycoprotein heterodimer that is linked by disulfide bonds. It is the fourth complement component to react in the classical pathway and it is also a reactant in the alternative complement pathway. C3 convertase splits C3 into C3b and C3a. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors for C3b. C3a is released into the surrounding fluids. It can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin. In blood, the C3a fragment is rapidly desarginated by serum carboxypeptidase N enzyme to generate C3a desArg. Both C3a and C3a desArg are not glycosylated. Applications: Suitable for use in ELISA. Western Blot and Immunoprecipitation have been reported in literature. Other applications not tested. Recommended Dilution: ELISA (capture): 2-6ug/ml, To obtain a linear standard curve, serial dilutions of C3a recombinant protein ranging from 39-2500pg/ml are recommended for each ELISA plate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice