046108
Histone H3, phosphorylated (Ser28) (Histone H3.1, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ) (PE)
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£685.00
- SKU:
- 046108
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by affinity chromatography
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Synthetic peptide corresponding to aa23-35 of human Histone H3 conjugated to KLH
- Klon:
- HTA28
- Format:
- Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-a (mixed linage kinase-like mitogen activated protein triple kinase a) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice