5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine)

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£850.00

SKU:: 045667
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: BSA-conjugated recombinant Hydroxymethylcytosine DNA.
Klon:: HMC-MA01
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation. Applications: Suitable for use in Immunocytochemistry, Dot Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Dot Blot: 1:5000 detected only 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA. Immunoprecipitation: Immunoprecipitates 5-Hydroxymethylcytosine DNA using chomatin from 293T cells overexpressing mTET1-CD. (Dr. D. Chen, Shanghai Institute of Biological Sciences) Immunocytochemistry: A representative lot was used by an independent laboratory to stain 5-Hydroxymethylcytosine DNA in cortical neurons from atg5-disrupted mouse. (Dr. D. Chen, Shanghai Institute of Biological Sciences) Optimal dilutions to be determined by the researcher. Control: Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice