045667-ML650
5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine) (MaxLight 650)
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£1,106.00
- SKU:
- 045667-ML650
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- BSA-conjugated recombinant Hydroxymethylcytosine DNA.
- Klon:
- HMC-MA01
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation. Applications: Suitable for use in Dot Blot, Immunoprecipitation. Recommended Dilution: Dot Blot: 1:5000 detected only 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA. Immunoprecipitation: Immunoprecipitates 5-Hydroxymethylcytosine DNA using chomatin from 293T cells overexpressing mTET1-CD. (Dr. D. Chen, Shanghai Institute of Biological Sciences) Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to stain 5-Hydroxymethylcytosine DNA in cortical neurons from atg5-disrupted mouse. (Dr. D. Chen, Shanghai Institute of Biological Sciences) Control: Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice