Tim-3 (T Cell Immunoglobulin and Mucin Domain Containing Protein-3, HAVcr-2, Hepatitis A Virus Cellular Receptor 2 Homolog)

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£364.00

SKU:: 045337
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse Tim-3.
Klon:: TI 339H
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Weitere Details:: Tim-3 is a Th1-specific cell surface protein. It is expressed selectively by differentiated CD4+Th1 and CD8+Tc1 cells, but is absent on CD4+Th2 and CD8+ Tc2 cells. The Tim gene family has an important role in the regulation of autoimmunity and allergies. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (direct or indirect): 1:2000-1:10,000 Western Blot: 1:2000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.