RANKL (TRANCE, ODF, OPGL, TNFSF11, CD254)

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£416.00

SKU:: 045196
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human RANKL.
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Weitere Details:: RANKL is an osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. Important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Plays an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Applications: Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: ELISA (direct and indirect): 1:2000-1:10,000 Flow Cytometry: 1:500-1:1000 Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.