Pix beta (Rho Guanine Nucleotide Exchange Factor 7, PAK-interacting Exchange Factor beta, p85SPR, COOL-1)

Größe

£479.00

SKU:: 045155
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to mouse beta-Pix SH3 domain.
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Weitere Details:: beta-PIX acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. It interacts with PAK kinases through the SH3 domain. It may function as a positive regulator of apoptosis and is involved in cell migration. It is expressed with high levels in the central nervous system. Applications: Suitable for use in ELISA, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: ELISA (Direct and Indirect): 1:5000-1:10,000 Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature Immunocytochemistry: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.