045133
PEDF (Recombinant) (Pigment Epithelium-derived Factor, Cell Proliferation-inducing Gene 35 Protein, EPC-1, Serpin F1)
Größe
£550.00
- SKU:
- 045133
- Reinheit:
- Purified by Protein A affinity chromotography from HEK 293 cell culture supernatant (≥95% SDS-PAGE).
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Human
- Immunogen:
- Human recombinant PEDF.
- Klon:
- Serpy-1-4
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
- Weitere Details:
- Pigment epithelium-derived factor (PEDF) is a 47kD secreted glycoprotein that belongs to the non-inhibitory serpin family group. PEDF is widely expressed in adult and fetal tissues, including brain, spinal cord, plasma, bone, prostate, pancreas, heart and lung. PEDF acts as an angiogenesis inhibitor with neurotrophic, immunomodulation and antitumor properties. It functions as anti-angiogenic agent by counterbalancing the proangiogenic effect of VEGF. PEDF is one of the most abundant proteins released by adipocytes and induces insulin resistance in adipocytes and human skeletal muscle cells. Recently, it has been reported that PEDF is sufficient to maintain the self-renewal of pluripotent human embryonic stem cells. Anti-PEDF (human), mAb (rec.) (Serpy-1-4) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice