NLRP3/NALP3 (NACHT-, LRR- and PYD-containing Protein 3, NLRP3, Cryopyrin, Cold Autoinflammatory Syndrome 1 Protein)

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£575.00

SKU:: 045088
Reinheit:: ≥95% (SDS-PAGE) from concentrated hybridoma tissue culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to mouse NLRP3/NALP3 (pyrin domain/aa1-93).
Klon:: Cryo-2
Format:: Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
Weitere Details:: The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA). Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and ChIP. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Immunocytochemistry: See J. Virol. 85, 13019 (2011) and Nat. Immunol. 13, 255 (2012) Immunohistochemistry: 1;200. See Nature 493, 67 (2012) Immunoprecipitation; In RIPA Buffer. See Mol. Cell 49, 331 (2012) ChIP: 2ug/DNA of 10e6 cells (see Nat. Immunol. 16, 859 (2015)) Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice