FOXP3 (Forkhead Box Protein P3, Scurfin)

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£371.00

SKU:: 044822
Reinheit:: Purified by Protein G affinity chromatography from hybridoma tissue culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse FOXP3.
Klon:: MF333F
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Weitere Details:: FOXP3 is involved in immune system responses. It functions as the master regulator in the development and function of regulatory T cells. FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX); also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. Applications: Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (direct or indirect): 1:2000-1:10,000 Immunohistochemistry (paraffin or frozen): 1:50-1:100 Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice