044629
Caspase-1 (Interleukin-1 beta Convertase, IL-1BC, Interleukin-1 beta-converting Enzyme, ICE)
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£557.00
- SKU:
- 044629
- Reinheit:
- Purified by Protein G affinity chromatography from concentrated hybridoma tissue culture supernatant. ≥95% (SDS-PAGE)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to mouse caspase-1.
- Klon:
- Casper-1
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
- Weitere Details:
- Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals. Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation) Immunohistochemistry (Paraffin): 1:500 Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Immunoprecipitation: 1:200 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice