042068
SMURF2, CT (SMURF2, E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2)
Größe
£696.00
- SKU:
- 042068
- Reinheit:
- Purified by ammonium sulfate precipitation
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 702-731 in the C-terminal region of human SMURF2. Species Sequence Homology: mouse, Xenopus.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Weitere Details:
- SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with SMAD1, SMAD2 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. It enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:2000 Immunohistochemistry (FFPE): 1:50-1:100 Immunofluorescence: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice