040792
RAD17, ID (RAD17, R24L, Cell cycle checkpoint protein RAD17, RF-C/activator 1 homolog)
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£696.00
- SKU:
- 040792
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-254 amino acids from the Central region of human RAD17.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Weitere Details:
- The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. Applications: Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Indirect Flow Cytometry: 1:10-50 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice