EIF4E (EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25kD subunit, mRNA cap-binding protein)

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£696.00

SKU:: 035012
Reinheit:: Purified by Protein A affinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human VGFR2
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Weitere Details:: EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Indirect Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.