Visinin-like Protein 1 (VSNL1, VILIP, VISL1, VLP-1, Hippocalcin-like Protein 3, HLP3)

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£544.00

SKU:: 031446
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to full length human VSNL1
Klon:: 3A9
Format:: Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol.
Weitere Details:: Visinin-like protein 1 (VSNL1) is a small Calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression (4, 5). It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamly of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the confomation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding. Visinin-like protein 1 is 191aa in size and has a molecular weight on SDS-PAGE of 22kD. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:500-1:1000 Immunohistochemistry: 1:500-1:1000 Western Blot: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice