TATA-binding Protein-associated Factor 2N (68kD TATA-binding Protein-associated Factor, TAF(II)68, TAFII68, RNA-binding Protein 56, RBP56, TAF15, TAF2N)

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£544.00

SKU:: 031433
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant proteiin corresponding to human TAF15 expressed in E. coli.
Klon:: 4D71
Format:: Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol
Weitere Details:: TAF15 is a member of a family of 3 closely related mammalian RNA binding proteins, the other members being FUS/TLS and EWSR1. TDP43 is a more distant relative of these three. The protein is also known as RBP56, for RNA binding protein 56kD (1). The Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name for this protein is TAF15. As with the other members of this family, TAF15 contains one copy of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and DNA. It also has a single Zinc Finger domain of the ZnF_RBZ subtype found in Ran binding proteins. Ran is a small G protein related to p21-Ras which regulates the import and export of proteins to the nucleus. Like TDP43, Fus/TLS and EWS, TAF15 is widely expressed in tissues and is localized primarily in the nucleus of cells (2). It was recently shown that point mutations in TAF15 may be associated with some familial forms of Lou Gehrig's disease, also known as amyotrophic lateral sclerosis or ALS (3). Fus/TLS and TDP43 protein mutations and aggregation have been implicated in various kinds of neurological disease including ALS (4). The HGNC name for this protein is TAF15. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Not recommended for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:500-1:1000 Western Blot: 1:1000; A suitable control tissue is HeLa cell lysates. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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