031429
Splicing Factor 3B Subunit 4 (SF3B4, SF3b50, Pre-mRNA Splicing Factor SF3b 49kD Subunit, Spliceosome-associated Protein 49, SAP49)
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£544.00
- SKU:
- 031429
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length recombinant protein corresponding to human SF3B4 expressed in E. coli.
- Klon:
- 3A1
- Format:
- Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol .
- Weitere Details:
- Splicing factor SF3B4, also known as SAP49, is a ubiquitously expressed protein found in the nuclei of eukaryotic cells (1). The protein is also known as splicing factor 3b, subunit 4, 49kDa SAP49, spliceosome-associated protein U2, Hsh49 and MGC108282. SF3B4 is one of four protein components of the U2 small nuclear ribonucleoprotein (snRNP) dependent splicing factor protein complex, the others three being SAP130, SAP145 and SAP155 (2). SF3B4 is one of the numerous proteins to contain one or more RNA recognition motifs. These are ~70 amino acids domains which bind single stranded RNA, and the SF3B4 protein has two of these. The function of the U2 snRNP dependent spliceosome complex is to regulate mRNA splicing. A rare human genetic disorder, Nager syndrome, is caused by haploinsufficiency of SF3B4 (3,4). The syndrome is variable in effect in different individuals but is classified as one of the acrofacial dysostoses which affect the face and limbs. Another study shows that Rodriguez acrofacial dysostosis may also be caused by point mutations in the SF3B4 gene (5). Recent studies suggest that upregulation of SF3B4 expression is an early stage biomarker of hepatocellular carcinoma (HCC), the most common form of cancer of the liver (6). Interestingly, knock down of SF3B4 expression may be beneficial therapeutically to HCC and other cancer patients as reduced levels of this protein inhibit alternate splicing of mRNA as activation of mRNA splicing is a feature of many cancer cells (6). Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:1000 Western Blot: 1:1000 Immunohistochemistry 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice