Adenylate Cyclase Type 3 (Adenylate Cyclase Type III, AC-III, Adenylyl Cyclase 3, AC3, Adcy3, Adenylate Cyclase Olfactive Type, ATP Pyrophosphate-lyase 3)

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£607.00

SKU:: 031349
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: C-terminal peptide of rat ACIII, PAAFPNGSSVTLPHQVVDNP with a Cys added to the N-terminus to allow coupling to KLH.
Format:: Supplied as a liquid in PBS, 0.03% sodium azide, 50% glycerol.
Weitere Details:: G-protein coupled receptors are a large and variable family of membrane proteins. On binding their specific ligand they activate specific members of the family of trimeric G-proteins which in turn activate other signaling enzymes. Adenylate cyclases are one of these and are activated by the GTP bound GαS subunits of trimeric G-proteins. Adenylate cyclases are responsible for the production of the important “second messenger” signaling molecule cyclic-AMP which in turn activates the cAMP dependent protein kinase. This kinase phosphorylates numerous substrate molecules on serine or threonine residues and so alters their activity. There are several different adenylate cyclase genes and protein products with each have distinctly different distribution patterns in cells and tissues. The type III adenylate cyclase enzyme is specifically localized in the membranes surrounding neuronal cilia, and is activated by specific G-protein coupled receptors also located in cilia. Neuronal cilia express a variety of other receptors types and mediators of other signaling pathways and appear to function as a unique and complex neuronal sensory structure. For examples, the somatostatin 3 receptor, neuropeptide Y 2 receptor and melanin concentrating hormone receptor 1 are localized in neuronal cilia and the sonic hedgehog and Wnt signaling pathway act on neurons primarily through neuronal cilia. The antibody works on mouse cells which express the same peptide and also on human cells, presumably because the corresponding peptide in the human AC3 sequence is the closely related peptide LATFPNGPSVTLPHQVVDNS. In line, 031349 binds full length transfected human ACIII on western blots. The antibody works well to identify neuronal cilia on human and rodent cells. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:2000-1:5000 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice