030153
E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3)
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£652.00
- SKU:
- 030153
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. coli.
- Klon:
- 13B231
- Format:
- Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.
- Weitere Details:
- The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent:independent apoptosis. Applications: Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Flow Cytometry: 1:200-1:400 ELISA: 1:10000 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice