FOXO1, phosphorylated (Thr24) (FKHR, Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead In Rhabdomyosarcoma, FOXO1A) (APC)

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£965.00

SKU:: 029480-APC
Anwendung:: WB
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic linear peptide corresponding to human FOXO1 phosphorylated at Thr24 (KLH).
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Weitere Details:: Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions and cell proliferation. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. Increased cell proliferation occurs when FOXO1 is inactivated when phosphorylated by Akt at 24aa. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: detects phospho-FOXO1 (Thr24) in HEK293 cell lysate untreated and BMP2-treated. Optimal dilutions to be determined by the researcher. Positive Control: HEK293 cell lysate untreated and BMP2-treated Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice