029453-BIOTIN
DEPTOR (DEP Domain-containing mTOR-interacting Protein, DEP Domain-containing Protein 6, DEPDC6) (Biotin)
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£927.00
- SKU:
- 029453-BIOTIN
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic linear peptide corresponding to the DEP domain, mTOR-interacting protein of human DEPTOR (KLH). Species sequence homology: Primate (99%), equine (99%), porcine (98%), canine (98%), rat (97%), mouse (96%).
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Weitere Details:
- DEPTOR was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. DEPTOR negatively regulates mTORC1 and mTORC2. It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival. Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: detected DEPTOR in HEK293 cell lysate. Optimal dilutions to be determined by the researcher. Positive Control: HEK293 cell lyaste Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice