PRLVV01

Lenti p24 Xpress Assay

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SKU:: PRLVV01
Lagerbedingungen:: 2-12°C
Hersteller:: Progen
Format:: Test Chips (rtu)
Weitere Details:: <b>Reliable and Automated p24 Titer in under 20 Minutes </b><div>Accurate lentiviral vector development depends on fast, dependable process monitoring. Traditional ELISA workflows can take several hours and require multiple manual steps, which slows progress upstream and downstream. </div><div>The microfluidic-based Lenti p24 Xpress Assay (Cat.# PRLVV01), runs on the <a target="_blank" href="https://www.progen.com/genspeed-r2-analyzer/prr2?_gl=1*m6zuw5*_up*MQ..*_ga*MTQxOTYzODExNS4xNzc4NTg4MzEz*_ga_Z1T7SM9YW5*czE3Nzg1ODgzMTMkbzEkZzEkdDE3Nzg1ODgzMzYkajM3JGwwJGgw" rel="noopener"> GENSPEED® R2 Analyzer (Cat.# PRR2)</a>and was developed to simplify and accelerate p24 quantification. With an automated, single sample workflow, it delivers rapid, consistent results designed for gene therapy environments in <20 minutes. </div><div><br></div><div><span style="letter-spacing: 0px;"><b>Performance Snapshot </b></span></div><div><table class="sw-text-editor-table"><tbody class="sw-text-editor-table__body"><tr class="sw-text-editor-table__row"><td class="sw-text-editor-table__col" style="width: 147px;">Parameter<div class="sw-text-editor-table__col-selector" style="height: 206px;" contenteditable="false"></div><br></td><td class="sw-text-editor-table__col" style="width: 349px;">Target Specification<div class="sw-text-editor-table__col-selector" style="height: 206px;" contenteditable="false"></div></td></tr><tr class="sw-text-editor-table__row"><td class="sw-text-editor-table__col">Assay time</td><td class="sw-text-editor-table__col"><20 minutes</td></tr><tr class="sw-text-editor-table__row"><td class="sw-text-editor-table__col">Sample type</td><td class="sw-text-editor-table__col">Crude or purified lentivirus</td></tr><tr class="sw-text-editor-table__row"><td class="sw-text-editor-table__col">Sample volume</td><td class="sw-text-editor-table__col">~5-15 µl (depending on sample type)</td></tr><tr class="sw-text-editor-table__row"><td class="sw-text-editor-table__col">Dynamic range</td><td class="sw-text-editor-table__col">~100-1,000,000 ng/ml</td></tr></tbody></table><span style="letter-spacing: 0px;"><br><b>Kit Components </b></span></div><div><span style="letter-spacing: 0px;">Lenti p24 Xpress Assay Kit – allows the measurement of 25 samples <br>25 × Test Chips (ready to use)<br>Solution A (ready to use)<span></span><br>Solution B (ready to use)<br>1 × Substrate cartridge<br>PROGEN Report Software Access <br>Quick start guide </span></div><div><span style="letter-spacing: 0px;"><b><br></b></span></div><div><span style="letter-spacing: 0px;"><b>Assay Principle</b></span></div><br><br><img alt="Genspeed R2 Analyzer" style="display: block; margin-left: auto; margin-right: auto; width: 70%;" src="https://www.progen.com/media/3e/5c/14/1776781862/p24-assay-principle.png?1777290855"><div><span style="letter-spacing: 0px;"><p class="MsoNormal" style="text-align:justify""><span lang="EN-US"" style="mso-ansi-language:EN-US"><br></span></p><p class="MsoNormal" style="text-align:justify""><span lang="EN-US"" style="mso-ansi-language:EN-US">The Lenti p24 Xpress Assay is a microfluidic test system, allowing the detection of p24 protein via a multi step immunochemical process: </span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-US" style="mso-ansi-language:EN-US">1. A biotin-labelled detection nanobody binds to the Lenti p24 capsid protein during initial dilution of the sample, forming an immune complex. </span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-US" style="mso-ansi-language:EN-US">2. After application to the Lenti p24 Xpress Assay Test Chip , the p24-detection nanobody-complex is bound by the p24 capture antibody printed on the test chip. </span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-US" style="mso-ansi-language:EN-US">3. Unbound detection nanobody is captured by immobilized recombinant p24 protein on the Test Chip. </span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-US" style="mso-ansi-language:EN-US">4. The addition of Strep-HRP and the substrate lead to a detection signal. </span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-US" style="mso-ansi-language:EN-US">5. Signal evaluation by the software enables accurate quantitative p24 analysis. </span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-US" style="mso-ansi-language:EN-US">The <b>PROGEN Report Software*</b> automatically evaluates the measured signals in comparison with an internal, batch specific standard curve based on recombinant p24 protein embedded in the system. Based on this calibration, the software calculates the quantitative p24 titer as well theoretical virus particle titer of the unknown sample and generates a comprehensive results report. As an additional part of the overall validity of the test system, each Lenti p24 Xpress Assay Test Chip contains a low and high level internal control, which verifies the correct sample flow through the chip. All control parameters are automatically incorporated into the software’s evaluation algorithm. <br></span></p><i><br></i></span></div><div><span style="letter-spacing: 0px;"><i><p class="MsoNormal"><span lang="EN-US" style="mso-ansi-language:EN-US">* Download access to the PROGEN Report Software is included with this product. Please note that the software is compatible with Windows operating systems only!</span></p><p class="MsoNormal"><span lang="EN-US" style="mso-ansi-language:EN-US"><br></span></p></i></span></div><div><span style="letter-spacing: 0px;"><i><b>Research Use Only (RUO)</b>. Not for use in diagnostic procedures. However please note, that the Genspeed R2 Analyzer is CE?marked and approved as an In-Vitro Diagnostic (IVD) medical device under Regulation (EU) 2017/746, when used in combination with GENSPEED IVD test kits. It is therefore, and only under these circumstances suitable for use within the IVD regulatory context.</i></span></div>

Versandbedingungen:: Blue Ice