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  2. Recombinant Proteins

62003

Keratin K8 (Rind, Mr 52.000)

Eine Lieferung in diese Region ist nicht möglich.

SKU:
62003
Zusätzliche Namen:
Cytokeratin 8
Reinheit:
> 95% (determined by SDS gelelectrophoresis)
Quelle:
Bovine liver
Lagerbedingungen:
Please refer to datasheet
Hersteller:
Progen
ABP:
No Import Docs
Weitere Details:
Protein standard for immunoblotting, immunization, and immunoassays. Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). - Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841 - Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
Versandbedingungen:
Blue Ice