MBS6508405
Tumor Necrosis Factor alpha (TNFa)
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- SKU:
- MBS6508405
- Zusätzliche Namen:
- [tumor necrosis factor isoform 2; Tumor necrosis factor; tumor necrosis factor; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a]
- Anwendung:
- WB
- Konzentration:
- ~3mg/ml
- Reinheit:
- Highly Purified
- Lagerbedingungen:
- May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||TNF-a Neutralization Assay:|1. Plate 2 x 10e4 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.|2. Incubate overnight at 37 degree C in humidified 5% CO2 incubator.|3. The next day, prepare serial dilutions of T9160-31 in medium containing 2ug/ml of actinomycin D, for a final volume of 50ul.|4. Add 50ul of mouse TNFa (50pg/ml final concentration) to each antibody dilution and incubate for 1 hour at 37 degree C.|5. Add 100ul of the antibody/antigen mixture to the pre-seeded L929B cells.|6. Incubate 24 hours at 37 degree C, 5% CO2.|7. Score wells for cytotoxicity.
- Hersteller:
- MyBiosource
- Host:
- Rat
- Reaktivitäten:
- Mouse
- Klon:
- [0.T.151]
- Uniprot:
- P06804
- Gendetails:
- [TNFa]