Alkaline Phosphatase (ALP) Activity Colorimetric Assay Kit(Method of NPP-AMP)

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£141.30

War £157.00

SKU:: E-BC-K1304-M
Physischer Zustand:: Plasma, Serum, Tissue
Lagerbedingungen:: 2-8[o]C Protect from light.
Hersteller:: Elabscience
Immunogen:: ALP
Format:: Colorimetric
Weitere Details:: Using 4-Nitrophenyl phosphate (4-NPP) as the substrate and 2-Amino-2-methyl-1-propanol (AMP) as the receptor substance for the phosphate group, alkaline phosphatase (ALP) catalyzes the reaction to generate free 4-nitrophenol (4-NP). The latter converts into a quinone structure in an alkaline solution and exhibits a darker yellow color. The activity of ALP in the sample can be calculated based on the rate of increase in absorbance at 405 nm.