BNUM0183
Cytochrome C (Mitochondrial Marker)(7H8.2C12), 1mg/mL
Eine Lieferung in diese Region ist nicht möglich.
- SKU:
- BNUM0183
- Zusätzliche Namen:
- CYC; CYCS; HCS; THC4
- Anwendung:
- WB, IHC-P
- Konzentration:
- 1 mg/ml
- Molekulargewicht:
- 15 kDa
- Quelle:
- Animal
- Lagerbedingungen:
- -20[o]C Avoid freeze/thaw cycles.
- Hersteller:
- Biotium
- Host:
- Mouse
- Reaktivitäten:
- Canine, Human, Mouse, Rat, Equine, Insect/Arthropod
- Buffer:
- PBS, no BSA, no azide|PBS, no BSA, no sodium azide
- Immunogen:
- Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
- Formulierung:
- PBS, no BSA, no azide
- Klon:
- 7H8.2C12
- Uniprot:
- P99999
- Synonyme:
- CYC;cytochrome c;HCS;THC4
- Weitere Details:
- Recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Versandbedingungen:
- Ambient