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ACT-1040

Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody

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£364.00

SKU:
ACT-1040
Anwendung:
WB
Konzentration:
10 mg/ml
Physischer Zustand:
Liquid
Molekulargewicht:
42 kDa
Spezies-Reaktivität:
Rabbit
Aufreinigung:
Chickens were immunized with purified actin from rabbit muscle emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using actin conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 ug/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles. Protect from light.
Hersteller:
Aves Labs
Host:
Chicken/Avian
Reaktivitäten:
Human, Mouse, Rat
Buffer:
Sodium phosphate (10 mM
Immunogen:
Actin purified from Rabbit muscle
Formulierung:
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent.
Format:
Mixture of IgY fraction and affinity-purified antibodies
Uniprot:
P63267
Synonyme:
ACT;ACTA3;ACTE;actin-like protein;actin, gamma 2, smooth muscle, enteric;actin, gamma-enteric smooth muscle;ACTL3;ACTSG;alpha-actin-3;Gamma-2-actin;MMIHS5;Smooth muscle gamma-actin;VSCM;VSCM1
Weitere Details:
Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes., Aves Labs
Versandbedingungen:
Blue Ice