HGF-R
Produktgrößen
100 ug
£674,12
103-M184-100UG
Über dieses Produkt
- SKU:
- 103-M184
- Zusätzliche Namen:
- Met; HGF; HGFR; Par4; c-Met; AI838057
- Anwendung:
- Western Blot
- Buffer:
- PBS
- translate.label.attr.clone:
- 6F7
- Klonalität:
- Monoclonal
- Weitere Details:
- HGF R, also known as Met (from N-methyl-N-nitro-N-nitroso-guanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfidelinked dimer composed of a 50 kDa extracellular A Alpha chain and a 145 kDa transmembrane B Beta chain. The extracellular domain (ECD) contains a seven bladed B Beta-propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like Eset domains, while the cytoplasmic region includes the tyrosine kinase domain. An alternately spliced form of mouse HGF R lacks a cytoplasmic juxtamembrane region important for regulation of signal transduction. The sema domain, which is formed by both the A Alpha and B Beta chains of HGF R, mediates both ligand binding and receptor dimerization. Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2containing molecules. HGF stimulation induces HGF R downregulation via internalization and proteasomedependent degradation. In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin A Alpha6/B Beta4, plexins B1, 2, 3, and MSP R/Ron (11 18). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (11 18). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion. Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells. Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers. Within the ECD, mouse HGF R shares 87%, 87%, and 94% amino acid sequence identity with canine, human, and rat HGF R, respectively.
- Formulierung:
- lyophilized
- Host:
- Rat
- Immunogen:
- Recombinant Mouse HGF-R extracellular domain
- Isotyp:
- IgG1
- Aufreinigung:
- Protein G Purified
- Reaktivitäten:
- Mouse
- Versandbedingungen:
- Ambient
- Lagerbedingungen:
- -20[o]C lyophilized. Avoid freeze/thaw cycles.
- Typ:
- Antibody: Monoclonal Antibody