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Antikörper

anti-AAV VP1/VP2/VP3, B1, mouse monoclonal antibody, liquid

Produktgrößen
200 ul
£176,00
690058-200UL
50 ug
£540,00
690058-50UG
Über dieses Produkt
SKU:
690058
Anwendung:
Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Purification, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
B1
Klonalität:
Monoclonal
Konzentration:
50 ug/ml
Weitere Details:
The B1 antibody reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a reduced degree with assembled viral particles. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281-93 (2000). Limited Use Label License: Research Use Only Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.
Formulierung:
PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA
Host:
Mouse
Immunogen:
AAV2 capsids
Isotyp:
IgG1
Aufreinigung:
Affinity Purified
Reaktivitäten:
Virus
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Please refer to datasheet
Größe:
50 ug
Typ:
Antibody: Monoclonal Antibody
  • Datenblatt des Herstellers:690058
Alignment of B1 epitopes in different AAV serotypes.
Dot blot analysis of denatured AAV2 VP2 capsids with B1 antibody (Cat. No. 690058) and ECL detection. Dot blot analysis was performed on 1E+10
5E+09 or 1E+09 denatured AAV2 capsids. The capsids were denatured for 10 min at 95°C. The nitrocellulose membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-AAV VP1/VP2/VP3
B1 (Cat. No. 690058) was diluted in blocking buffer (antibody concentration 100 ng/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG HRP was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Plus Western Blotting Substrate.
Western blot analysis of recombinant AAV2 capsid proteins (Cat. No. 640823