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Antikörper

anti-Synaptophysin, SY38, mouse monoclonal antibody

Produktgrößen
50 ug
£309,00
61012-50UG
Über dieses Produkt
SKU:
61012
Zusätzliche Namen:
Synaptophysin, Major synaptic vesicle protein p38, SYP
Anwendung:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
SY38
Klonalität:
Monoclonal
Konzentration:
50 ug/ml
Weitere Details:
SY38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid. Polypeptide reacting: 38 kDa transmembrane glycoprotein of presynaptic vesicles. SY38 binds to a cytoplasmatic domain of synaptophysin. The epitope was located to a flexible segment in the center of the repeat structure (Knaus and Betz, 1990). Tested cultured cell lines: rat PC-12 cell line. Knaus, P. & Betz, H. Mapping of a dominant immunogenic region of synaptophysin, a major membrane protein of synaptic vesicles. FEBS Lett. 261, 358-360 (1990).
Formulierung:
Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Host:
Mouse
Immunogen:
Synaptophysin from presynaptic vesicles, prepared from bovine brain
Isotyp:
IgG1
Physischer Zustand:
Lyophilized
Aufreinigung:
Affinity Purified
Reaktivitäten:
Bovine, Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
2-8[o]C reconstituted. Aliquot. Avoid freeze/thaw cycles.
Typ:
Antibody: Monoclonal Antibody
  • Datenblatt des Herstellers:61012
Western blot analysis of mouse brain lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 ug mouse brain lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal
SY38 (Cat. No. 690012) was diluted in blocking buffer (antibody concentration 0.05 ug/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse
HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
Western blot analysis of human brain lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 ug human brain lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal