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Assay-Kits & ELISAs

ImmPRESS[R]-AP Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Detection Kit, Alkaline Phosphatase

Produktgrößen
15 ml
£416,00
MP-5444-15ML
Über dieses Produkt
SKU:
MP-5444
Zusätzliche Namen:
ImmPRESS Enzyme Polymer Reagents [Detection Systems ]
Anwendung:
IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunohistochemistry
Konjugat:
AP
Weitere Details:
The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. This secondary antibody can be used to stain mouse tissue because it is specifically adsorbed to remove cross-reacting antibodies of closely-related species.Features:Non-biotinEnhanced accessibility to nuclear and membrane antigensReady-to-use in a convenient dropper bottleShorter assay timeSimplified multiple labellingThe ImmPRESS-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens, and greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications. Immunostaining ProtocolThe staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.
Host:
Goat
Immunogen:
Rat IgG (Mouse Adsorbed) Polymer Kit
Aufreinigung:
Adsorbed
Versandbedingungen:
Ambient
Lagerbedingungen:
2-8[o]C
Typ:
Assay Kits: IHC Kits