Click-&-Go[R] Plus 555 OPP
Produktgrößen
1 kit
£467,00
CCT-1494-1KIT
Über dieses Produkt
- SKU:
- CCT-1494
- Anwendung:
- Conjugation/Labeling
- Weitere Details:
- Click-&-Go® Plus 555 OPP Protein Synthesis Assay Kit enables sensitive, and non-radioactive chemical method to image and affinity-purify nascent proteins in cells and in animals, based on an alkyne analog of puromycin, O-propargyl-puromycin (OPP). OPP is an analog of puromycin that contains a terminal alkyne group, enters the acceptor site of ribosomes and incorporates into nascent polypeptide chains. OPP forms covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized through copper(I)-catalyzed azide-alkyne cycloaddition. Unlike methionine analogs, OPP does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms. This strategy should have broad applicability for imaging protein synthesis and for identifying proteins synthesized under various physiological and pathological conditions in vivo.
- Versandbedingungen:
- Ambient
- Lagerbedingungen:
- 2-8[o]C
- Hersteller:
- Vector - Click Chemistry Tools
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
- Datenblatt des Herstellers:Click-Go-Plus-555-OPP
