AZDye 647 Picolyl Azide
Produktgrößen
1 mg
£301,00
CCT-1300-1MG
5 mg
£769,00
CCT-1300-5MG
25 mg
£2293,00
CCT-1300-25MG
Über dieses Produkt
- SKU:
- CCT-1300
- Anwendung:
- Conjugation/Labeling
- Konjugat:
- AZDye™ 647
- Weitere Details:
- AZDye™ 647 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported. In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol. In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging. AZDye™ 647 Picolyl Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
- Molekulargewicht:
- 1061.21 (protonated)
- Versandbedingungen:
- Ambient
- Lagerbedingungen:
- -20[o]C desiccated. Avoid freeze/thaw cycles.
- Hersteller:
- Vector - Click Chemistry Tools
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
- Datenblatt des Herstellers:AZDye-647-Picolyl-Azide
