Staphylococcus aureus Enterotoxin B (SEB) (HRP)
Produktgrößen
100ul
£965,00
S7965-35B-HRP-100UL
Über dieses Produkt
- SKU:
- S7965-35B-HRP
- Anwendung:
- Western Blot
- translate.label.attr.clone:
- 3G314
- Klonalität:
- Monoclonal
- Weitere Details:
- Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30kD. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their aa sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of these substances. Enterotoxins are Superantigens, inducing polyclonal T cell activation by binding to the TCR and to the alpha chain of the MHC II molecule simultaneously. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Recombinant S. aureus enterotoxin B expressed in E. coli.
- Isotyp:
- IgG1
- Physischer Zustand:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Horseradish peroxidase (HRP).
- Reinheit:
- Purified by Protein G affinity chromatography from ascites. ≥95% (SDS PAGE).
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes Staphylococcus aureus Enterotoxin B (SEB). Does not crossreact with S. aureus enterotoxins A, C, D and E.
- Quelle:
- S.aureus
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody