Prothrombin (Not for Export EU)
Produktgrößen
1ml
£806,00
P9115-08E-1ML
Über dieses Produkt
- SKU:
- P9115-08E
- Klonalität:
- Polyclonal
- Weitere Details:
- Prothrombin (factor II, F.II) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of prothrombin in plasma is ~100ug/ml (~1.4uM). Prothrombin is a single chain molecule with a molecular weight of 72kD. Prothrombin consists of a catalytic domain followed by two kringle structures and an N-terminal domain containing 10 g-carboxy-glutamic acid (gla) residues. These gla residues allow prothrombin to bind to membranes that contain acidic phospholipids in a calcium dependent manner. The binding to membranes is required for effective presentation of prothrombin as a substrate for activation by the prothrombinase complex, which consists of activated factor X, activated cofactor V and calcium on phospholipid membrane. Activation by prothrombinase occurs by sequential cleavage after residue Arg320 then after Arg271 to produce the active protease a-thrombin (37kD) and the byproduct prothrombin fragment 1.2 (35kD). The product thrombin further cleaves prothrombin fragment 1.2 after residue Arg155 into individual prothrombin fragments 1 and 2. The activity of a-thrombin in plasma is inhibited primarily by antithrombin and the rate of inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin. Other physiological inhibitors of thrombin in the absence of heparin include a2macroglobulin and a1antitrypsin (1-3). Factor II also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in Factor II leads to various forms of thrombosis and dysprothrombinemia. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Sheep
- Immunogen:
- Mouse prothrombin.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid.
- Reinheit:
- Serum
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes mouse prothrombin.
- Quelle:
- mouse
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody