c-Phospholipase A2 (Cytosolic PLA2, cPLA2)
Produktgrößen
1mg
£863,00
P4074-03M-1MG
Über dieses Produkt
- SKU:
- P4074-03M
- Anwendung:
- Western Blot
- Klonalität:
- Polyclonal
- Weitere Details:
- Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and the release of arachidonic acid. cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity. Calcium binding to the Nterminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane. Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at serine 505 and serine 727 stimulates its catalytic activity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml, yields a positive response with 10ug of Raw cell line using alkaline phosphatase detection method. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa152-179 of mouse cPLA2. Sequence: D-Q-E-K-T-F-R-Q-Q-R-K-E-N-I-K-E-N-M-K- K-L-L-G-P-K-K-S-E.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Reinheit:
- Purified by ammonium sulfate precipitation.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes mouse cPLA2.
- Quelle:
- mouse
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody