Ogg1 (8-Oxoguanine DNA Glycosylase) (Not for Export EU)
Produktgrößen
100ul
£704,00
O5700-10-100UL
Über dieses Produkt
- SKU:
- O5700-10
- Klonalität:
- Polyclonal
- Weitere Details:
- Human 8-Oxoguanine DNA Glycosylase (hOGG1) is involved in the excision of 7, 8-dihydro-8-oxoguanine produced as a result of DNA damage. This gene has been localized on human chromosome 3p25. Several forms of hOGG protein have been cloned differing in lengths (351-424 aa) (refs 1). Full length human OGG1 fusion protein with N-terminal His-tag was expressed in E. coli and purified using Nickel-affinity support. Purified hOGG1 was injected into rabbits to produce antibodies. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000. Deytects a band at ~38kD from human cells and tissues. ELISA: 1:10,000-50,000. Using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Full length human OGG1 fusion protein with N-terminal Hs-tag expressed in E. coli.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid in PBS, 0.05% azide, before the addition of glycerol to 40%.
- Reinheit:
- Serum
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human OGG1.
- Quelle:
- human
- Lagerbedingungen:
- 4[o]C (-20[o]C Glycerol)
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody