Organic Anion Transporter 3 (OAT3) (Not for Export EU)
Produktgrößen
100ul
£704,00
O3003-21-100UL
Über dieses Produkt
- SKU:
- O3003-21
- Klonalität:
- Polyclonal
- Weitere Details:
- Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Recently OAT3 (rat 536-aa, human 568 aa) has been cloned that is most closely related to OAT1 (~49% identity). Rat OAT3 is expressed in the liver, brain, kidney and eye. OAT3 also mediates Na-independent uptake of several organic anions (PAH, ochratoxin, and estrone sulfate, etc). OAT3 shows 92% homology with mouse Roct (reduced in osteosclerosis transporter), possibly an ortholog of rat OAT3. As compared to OAT1-2, strong expression of OAT3 in brain suggests its role in removing organic anion in the brain. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL. ELISA: 1:10,000-50,000. Coat ELISA plates at 1ug/ml of Control Peptide. Optimal dilutions to be determined by the researcher. Control Peptide: O3003-22 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- A 16 AA Peptide sequence near the cytoplasmic, N-terminus of rat OAT3 (1), coupled to KLH
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid, 0.05% sodium azide, 40% glycerol.
- Reinheit:
- Serum
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes OAT3. No significant sequence homology is detected with other OATs or other proteins. Species crossreactivity: Rat. Species sequence homology: mouse-93%; human and rabbit -87%.
- Quelle:
- rat
- Lagerbedingungen:
- 4[o]C (-20[o]C Glycerol)
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody